The Impact of Diet on the Gut-Microbiota-Brain Axis

Inclusion Criteria:

• Be able to give written informed consent.

• Be between 18 and 50 years of age.

• Have a body mass index (BMI) between 18.5-29.9 Kg/m2.

• Be in generally good health as determined by the investigator.

Exclusion Criteria:

• Are less than 18 and greater than 50 years of age.

• Have a BMI below 18.5 or above 29.9 Kg/m2.

• Have a significant acute or chronic coexisting illness [cardiovascular,gastrointestinal (GI) [to include functional GI disorders, inflammatory boweldisease, coeliac disease, lactose intolerance, food allergies], immunological,psychiatric [to include formal or as determined by MINI Psychiatric interview,diagnosis of current major depression, anxiety disorder, bipolar spectrum disorder,schizophrenia, other DSM-IV Axis I disorder], neurodevelopmental disorders,immunological, metabolic disorders [to include type I or II diabetes], or anycondition which contraindicates, in the investigators judgement, entry to the study,

• Have a condition or taking a medication that the investigator believes wouldinterfere with the objectives of the study, pose a safety risk, or confound theinterpretation of the study results; all psychoactive medications [to includeanxiolytics, antipsychotics, antidepressants, anticonvulsants, centrally actingcorticosteroids, and opioid pain relievers), laxatives, enemas, antibiotics,anti-coagulants, over-the counter non-steroidal anti-inflammatories (NSAIDS).Subjects should have a wash-out period of 4 weeks.

• Current prebiotic or probiotic supplement use (a wash-out period of 4 weeks aftercessation will allow entry to the study).

• Females who are peri-menopausal, menopausal or post-menopausal.

• Females who are pregnant or planning a pregnancy, or lactating.

• Participants who are not fluent in English.

• Are colour blind.

• Have dyslexia or dyscalculia.

• Are a current habitual daily smoker.

• Individuals who, in the opinion of the investigator, are considered to be poorattendees or unlikely for any reason to be able to comply with the trial.

• Subjects receiving treatment involving experimental drugs. If the subject has beenin a recent experimental trial, these must have been completed not less than 30 daysprior to this study.

• Have a malignant disease or any concomitant end-stage organ disease.

• Have completed a study in our laboratory in the past 4 years.