Fasting Mimicking Diet and Autophagy

Inclusion Criteria:

• Ability and willingness to provide written informed consent;
• Ability and willingness to perform the study tests and adhere to study protocol (tothe best of the participant's knowledge);
• BMI 20-35 kg/m2 (inclusive) at screening;

Exclusion Criteria:

• Diabetes treatment other than diet or metformin monotherapy;
• History of gastric bypass;
• Subjects with recent weight loss (>5%), use of weight loss medication, participated ina weight loss program in the past 3 months;
• Type 1 diabetes (based on medical history provided at screening);
• Use of immune suppression drugs;
• Contraindication for study foods (special food needs and allergy);
• Women who are pregnant;
• Alcohol dependency (alcohol intake greater than two drinks per day for women and threedrinks per day for men).
• Has any medical disease or condition that, in the opinion of the principalinvestigator (PI) or appropriate study personnel, precludes study participation* (*Including acute, subacute, intermittent or chronic medical disease or condition thatwould place the subject at an unacceptable risk of injury, render the subject unableto meet the requirements of the protocol, or may interfere with the evaluation ofresponses or the subject's successful completion of this trial);